解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::The effect of citrinin on Ca2+ transport was studied in isolated kidney cortex and liver mitochondria, and baby hamster kidney cultured cells. The mycotoxin significantly inhibited the activity of 2-oxoglutarate and pyruvate dehydrogenases in both kidney cortex and liver mitochondria. Citrinin promoted a decrease in t...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290130110
更新日期:1995-03-01 00:00:00
abstract::The possibility that inositol lipid metabolism is related to nuclear events accompanying steroid hormone action has been investigated by comparing lipid phosphorylation and breakdown in normal rat liver nuclei and in hypo- and hypercortisolemic conditions. Lipid phosphorylation in vitro showed the presence of diacylgl...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290120308
更新日期:1994-09-01 00:00:00
abstract::The effects of either radiation or hyperthermia on the differentiation potential of NG108-15, a neuroblastoma-glioma hybrid cell line, were studied. After radiation and hyperthermia, the outgrowth of neurites from NG108-15 cells was potentiated, and polarizing current and voltage pulses induced a distinct action poten...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290120209
更新日期:1994-06-01 00:00:00
abstract::A previous study showing that ethanol (ETOH) blocked [3H]choline incorporation into phosphatidylcholine (PC) suggested an inhibition of PC biosynthesis in human leukemic monocyte-like U937 cells. The mechanism of the inhibitory action of ETOH was investigated. Cells were pulsed with [3H]choline for 30 min and chased i...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290120107
更新日期:1994-01-01 00:00:00
abstract::We studied the effect of PTH (10-100 nM) on transductive mechanisms (adenylate cyclase activity, Ca2+ metabolism, IP3 levels) in cell cultures derived from normal and otosclerotic human bone fragments. The cultured cells were osteoblast-like but with calcitonin-receptors still present and with PTH receptors coupled wi...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290110406
更新日期:1993-12-01 00:00:00
abstract::Beta-glucosidase activity was evaluated in situ by means of fluorogenic probes in normal human fibroblasts and fibroblasts from homozygous carriers of the Gaucher trait. Probe internalization, targeting to lysosomes and post-cleavage probe retention were the primary concerns. Internalization and targeting were attempt...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290110304
更新日期:1993-09-01 00:00:00
abstract::The rat osteosarcoma cell line UMR-106-01 has an osteoblast-like phenotype. When grown in monolayer culture these cells transport inorganic phosphate and L-alanine via Na(+)-dependent transport systems. Exposure of these cells to a low phosphate medium for 4 h produced a 60-70 per cent increase in Na(+)-dependent phos...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290110207
更新日期:1993-06-01 00:00:00
abstract::The main purpose of the present investigation was to study the effect of cloturin on aerobic glycolysis, endogenous and exogenous respiration and the level of ATP in both Ehrlich ascites carcinoma (EAC) and P388 murine leukaemia cells incubated in vitro. Also its effect on the level of total (T-SH) and non-protein (NP...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290100208
更新日期:1992-06-01 00:00:00
abstract::Proliferating-cell nuclear antigen (PCNA), also known as cyclin, is synthesized in proliferative cells and recently was identified as DNA polymerase-delta auxiliary protein. In this paper, the association of PCNA to the proliferative cells of plants was analysed using both autoantibodies to PCNA obtained from a patien...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290100209
更新日期:1992-06-01 00:00:00
abstract::In response to concanavalin A, cytoplasmic calcium movement was observed in human platelets, both in the presence of 1 mM Ca2+ or 1 mM EGTA in the medium. Concanavalin A also caused the activation of inositide turnover and the production of inositol phosphates, suggesting that activation of phospholipase C occurs. The...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290100109
更新日期:1992-03-01 00:00:00
abstract::The biochemical and quantitative cytochemical assays of the activity of uridine diphosphoglucose dehydrogenase (UDPG-D) have produced perplexing results. It is now shown that the perplexity may be due to the possibility that the coenzyme (NAD) required for UDPG-D activity, may be acting as a substrate for a second deh...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290090207
更新日期:1991-04-01 00:00:00
abstract::A method for the quantification of energy consuming processes described by Siems et al. for reticulocytes and by Müller et al. for ascites tumour cells was applied to balance the ATP-consumption of isolated rat hepatocytes. On the basis of decreased coupled respiration rates following the specific inhibition of energy...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290080407
更新日期:1990-10-01 00:00:00
abstract::The immunological induction of arthritis in the knee of the rabbit is well established as a model for human rheumatoid arthritis. It has the special advantage of allowing the development of the condition, and the effect of disease-modifying agents, to be followed. Attention has been focussed on the activity of glucose...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290080406
更新日期:1990-10-01 00:00:00
abstract::The subcellular distribution of newly absorbed iron in isolated mouse duodenal enterocytes was investigated by analytical subcellular fractionation using sucrose density gradient centifugation. Two major peaks of mucosal 59Fe activity were observed: one soluble and one particulate (density 1.18-1.20 g ml-1). The latte...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290080205
更新日期:1990-04-01 00:00:00
abstract::Pi depletion of proximal tubule cells isolated from mouse kidney results in a decrease in the cell content of fructose-2,6-bisphosphate and an increase in the rate of gluconeogenesis from pyruvate, malate and succinate. Gluconeogenesis from glycerol is unaffected by Pi depletion. Introduction of fructose-2,6-bisphosph...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290080106
更新日期:1990-01-01 00:00:00
abstract::Rat liver nonparenchymal cells (NPC) were prepared by pronase digestion and purified on discontinuous gradients on Nycodenz. Morphological and biochemical characterization of cell suspensions showed that they were free of contamination by hepatocytes. We have confirmed the usefulness of pyruvate kinase activity in mon...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290070406
更新日期:1989-10-01 00:00:00
abstract::Effects of methotrexate (MTX) on mitochondrial oxidative metabolism and ion transport were studied. MTX decreases the membrane potential (delta psi) upon energization of the mitochondrial membrane by NAD+-linked substrates and decreases the amplitude and velocity of swelling induced by glutamate and alpha-ketoglutarat...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290070208
更新日期:1989-04-01 00:00:00
abstract::The activity and hormonal regulation of NAD- and NADP-linked isocitrate dehydrogenase (EC.1.1.1.41 and EC.1.1.1.42, respectively) in the brain, liver and kidney cortex of female rats of various ages was investigated. The activity of NAD-ICDH of brain was greater than extramitochondrial (-c) or intramitochondrial (-m) ...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290060308
更新日期:1988-07-01 00:00:00
abstract::The study of the influence of the age of the animals (13 to 53 weeks) on the rate of ethanol metabolism in vivo and the total activity of liver alcohol dehydrogenase and microsomal ethanol oxidizing system showed a progressive decline with age. These effects were observed concomitantly with a diminution in the content...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290060103
更新日期:1988-01-01 00:00:00
abstract::Metabolism of arabinose 5-P, ribose 5-P and glucose 6-P in permeabilized and resealed Morris hepatoma 5123TC cells was investigated by measuring the contribution of these compounds to nucleic acid biosynthesis. The level of [14C]-arabinose (non-phosphorylated) incorporation into nucleic acids was slight, presumably du...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290050408
更新日期:1987-10-01 00:00:00
abstract::Extracellular acylglycerols are hydrolysed by lipases active at the surface of intact fat cells isolated from rat or human adipose tissue. During short-term incubation, rat fat cells hydrolyse di-[3H]oleyl-[14C]glycerol at a rate of 70 +/- 7.7 mU/10(6) cells (mean +/- S.E.) versus 440 +/- 62 mU/10(6) cells for the hyd...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290050304
更新日期:1987-07-01 00:00:00
abstract::Nuclear, chromatin and microsomal fractions were isolated from hepatocytes prepared from rats injected with [32P]O4(2-) and killed subsequently at times between 1 and 48 h. Specific activities of the total phospholipids (PL) were determined for each subcellular fraction. The major points noted were the initial specifi...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290040409
更新日期:1986-10-01 00:00:00
abstract::In murine thymocytes cyclic nucleotide phosphodiesterase is represented by cAMP- and cGMP-specific forms. cAMP and cGMP phosphodiesterase activities showed anomalous kinetic behaviour indicative of 'low' and 'high' affinity enzyme forms. Sucrose density gradient centrifugation resolved only 'low' affinity forms of cAM...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290040307
更新日期:1986-07-01 00:00:00
abstract::The aim of this study was to investigate the possibility of enhancing the yield of tissue plasminogen activator (tPA) from two epithelial cell lines of normal (non-malignant) derivation grown in tissue culture. The three agents used in this investigation were chosen because of their proven enhancing effect on analogou...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290040109
更新日期:1986-01-01 00:00:00
abstract::Rat liver mitochondria were stored at 0-4 degrees C for several days using an appropriate medium and energy source. The elimination of the majority of microsomes and lysosomes, that normally contaminate isolated mitochondria, had a positive effect in preservation of respiratory control, P:O ratio, and monoamine oxidas...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290030407
更新日期:1985-10-01 00:00:00
abstract::The levels of polyamines and their synthesizing enzymes in squamous cell carcinoma of prostate implanted in intact as well as castrated male rats were determined after certain hormonal manipulations. The tumour was found to grow with an identical rate in non-castrated and castrated rats. Polyamine content and activiti...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290030305
更新日期:1985-07-01 00:00:00
abstract::Nuclear T3 specific binding sites were characterized by Scatchard analyses of L-125I-T3 binding to nuclei extracted from freshly isolated and 1, 2 and 6 day-cultured hepatocytes. The results demonstrate a marked decrease in T3 binding capacity of nuclei extracted from 1 day-cultured cells followed by an almost complet...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290030203
更新日期:1985-04-01 00:00:00
abstract::A method has been developed to improve the quality of autoradiography. In addition to using high specific activity thymidine (3HTdr), low temperature exposure and scintillation fluid, FdUR was added to the cell suspension during incubation. It led to an increased amount of 3HTdr incorporation by the cells. By this met...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290030211
更新日期:1985-04-01 00:00:00
abstract::Pig thyroid Golgi vesicles incubated in a suitable medium were able to concentrate iodide from the medium. This trapping required the integrity of the vesicles, was time- and temperature-dependent, and was inhibited by a competitive inhibitor of iodide active transport (perchlorate), suggesting a facilitated transport...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290030106
更新日期:1985-01-01 00:00:00
abstract::Specific binding of a fully biologically active 125I-gonadotrophin releasing hormone (GnRH) to isolated anterior pituitary cells is time dependent, saturable and the concentration dependent binding curves exhibit positive cooperativity. Binding to intact or solubilized plasma membranes and an affinity purified GnRH re...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290020405
更新日期:1984-10-01 00:00:00
abstract::Analysis of free nucleotide composition of both avian thrombocytes and pig platelets showed quantitative differences in the level of adenine nucleotides. 3H-adenine taken up by turkey thrombocytes was metabolized mainly to adenine nucleotides was not released after thrombin action. Thrombin liberated non-radioactive a...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290020310
更新日期:1984-07-01 00:00:00
abstract::Rat intestinal epithelial cells were isolated by EDTA-chelation, combined with gentle shaking (modified Weiser procedure) or with strong longitudinal vibration (Harrison/Webster procedure). Both methods yield large numbers of viable cells and are relatively easy to use. Electronmicroscopical and biochemical data indic...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290020208
更新日期:1984-04-01 00:00:00
abstract::The new glass ionomer dental cement, ChemFil, was found to be moderately cytotoxic when freshly mixed in vitro. This was assessed by a reduction in fibroblast and macrophage counts following 24 h exposure to the material, and by alterations in enzyme levels and enzyme staining kinetics relative to control cultures. Te...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290020112
更新日期:1984-01-01 00:00:00
abstract::Using several physical techniques the surface of articular cartilage has been reported to be structurally different from the deeper layers. In this paper using immunohistochemical methods, the surface has been shown to contain a characteristically different collagen, Type I in contrast to Type II which is the major co...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290010304
更新日期:1983-10-01 00:00:00
abstract::The spreading of HeLa cells on plastic substratum is mediated by fibronectin-depleted foetal calf serum but not by fibronectin isolated by gelatin-Sepharose affinity chromatography. The same is true for freshly explanted chick embryonic chondrocytes. In contrast, BHK cell spreading exceeds 67% after 120 min at 37 degr...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290010208
更新日期:1983-07-01 00:00:00